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u2os cell line  (ATCC)


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    ATCC u2os cell line
    ( A–B ) Measurement of total Orai1 levels in the plasma membrane of STX11-depleted cells by flow-cytometry. <t>U2OS</t> cells stably expressing Orai1-BBS-YFP were transduced with Scr (black) or STX11 (red) shRNA, stimulated with 1 µM TG (broken lines) or control (solid lines) and incubated with alpha-bungarotoxin alexa fluor 647 (BTX-AF647). Total Orai1-YFP levels ( A ) and BTX-AF647 binding to surface Orai1 ( B ) were measured where binding to wildtype U2OS cells was used as the background fluorescence. N=3 ( C ) Representative quantitative PCR to assess the efficiency of knockdown in shRNA-treated Orai1-BBS-YFP expressing U2OS cells from ( A–B ). Total RNA extracted from cells treated with STX11 shRNA was subjected to qPCR analysis using Taqman probes for STX11. Data were normalized to RPL30 housekeeping gene. ( D–E ) Measurement of thapsigargin (TG) induced SOCE in U2OS cells treated with STX11 RNAi in ( A–B ). ( D ) Representative averaged traces of Fura-2 calcium imaging assay performed on cells in panels ( A–B ). ( E ) Bar plot showing relative mean % SOCE ± SE from three independent experiments in ( D ). Figure 2—figure supplement 2—source data 1. Folder containing the original flow cytometry files and gating strategy for . Figure 2—figure supplement 2—source data 2. Numerical data underlying the quantitative analyses represented in .
    U2os Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 8550 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/u2os+cell+line/U-2+OS/pmc13679927-110-7-11
    Average 99 stars, based on 8550 article reviews
    u2os cell line - by Bioz Stars, 2026-11
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    Images

    1) Product Images from "Syntaxin11 deficiency inhibits CRAC channel priming to suppress cytotoxicity and gene expression in T lymphocytes independent of membrane trafficking"

    Article Title: Syntaxin11 deficiency inhibits CRAC channel priming to suppress cytotoxicity and gene expression in T lymphocytes independent of membrane trafficking

    Journal: eLife

    doi: 10.7554/eLife.111335

    ( A–B ) Measurement of total Orai1 levels in the plasma membrane of STX11-depleted cells by flow-cytometry. U2OS cells stably expressing Orai1-BBS-YFP were transduced with Scr (black) or STX11 (red) shRNA, stimulated with 1 µM TG (broken lines) or control (solid lines) and incubated with alpha-bungarotoxin alexa fluor 647 (BTX-AF647). Total Orai1-YFP levels ( A ) and BTX-AF647 binding to surface Orai1 ( B ) were measured where binding to wildtype U2OS cells was used as the background fluorescence. N=3 ( C ) Representative quantitative PCR to assess the efficiency of knockdown in shRNA-treated Orai1-BBS-YFP expressing U2OS cells from ( A–B ). Total RNA extracted from cells treated with STX11 shRNA was subjected to qPCR analysis using Taqman probes for STX11. Data were normalized to RPL30 housekeeping gene. ( D–E ) Measurement of thapsigargin (TG) induced SOCE in U2OS cells treated with STX11 RNAi in ( A–B ). ( D ) Representative averaged traces of Fura-2 calcium imaging assay performed on cells in panels ( A–B ). ( E ) Bar plot showing relative mean % SOCE ± SE from three independent experiments in ( D ). Figure 2—figure supplement 2—source data 1. Folder containing the original flow cytometry files and gating strategy for . Figure 2—figure supplement 2—source data 2. Numerical data underlying the quantitative analyses represented in .
    Figure Legend Snippet: ( A–B ) Measurement of total Orai1 levels in the plasma membrane of STX11-depleted cells by flow-cytometry. U2OS cells stably expressing Orai1-BBS-YFP were transduced with Scr (black) or STX11 (red) shRNA, stimulated with 1 µM TG (broken lines) or control (solid lines) and incubated with alpha-bungarotoxin alexa fluor 647 (BTX-AF647). Total Orai1-YFP levels ( A ) and BTX-AF647 binding to surface Orai1 ( B ) were measured where binding to wildtype U2OS cells was used as the background fluorescence. N=3 ( C ) Representative quantitative PCR to assess the efficiency of knockdown in shRNA-treated Orai1-BBS-YFP expressing U2OS cells from ( A–B ). Total RNA extracted from cells treated with STX11 shRNA was subjected to qPCR analysis using Taqman probes for STX11. Data were normalized to RPL30 housekeeping gene. ( D–E ) Measurement of thapsigargin (TG) induced SOCE in U2OS cells treated with STX11 RNAi in ( A–B ). ( D ) Representative averaged traces of Fura-2 calcium imaging assay performed on cells in panels ( A–B ). ( E ) Bar plot showing relative mean % SOCE ± SE from three independent experiments in ( D ). Figure 2—figure supplement 2—source data 1. Folder containing the original flow cytometry files and gating strategy for . Figure 2—figure supplement 2—source data 2. Numerical data underlying the quantitative analyses represented in .

    Techniques Used: Clinical Proteomics, Membrane, Flow Cytometry, Stable Transfection, Expressing, Transduction, shRNA, Control, Incubation, Binding Assay, Fluorescence, Real-time Polymerase Chain Reaction, Knockdown, Analysis, Imaging


    Figure Legend Snippet:

    Techniques Used:

    Related Articles

    Cell Culture:

    Article Title: Cytoplasmic recapping commences the translation of a micropeptide, MARS from MALAT1 after the removal of an inhibitory RNA G-quadruplex
    Article Snippet: .. U2OS cell line was purchased from ATCC and cultured and maintained in an atmosphere of 5% CO 2 at 37°C in DMEM High glucose (Gibco) supplemented with 10% FBS (Himedia) and 1% Penicilin-Streptomycin cocktail (Gibco). .. For plasmid transfection in U2OS cells, we use JetPrime transfection reagent (PolyPlus) following the manufacture’s instruction.

    Article Title: Group-based learning on label-free phase-contrast images across dose and exposure time improves bioactive compound classification
    Article Snippet: .. Cell culture and treatments The U2OS cell line was obtained from ATCC and cultured in McCoy’s 5 A Medium supplemented with 10% fetal bovine serum (FBS) and penicillin/streptomycin. ..

    Expressing:

    Article Title: ER tethering and active transport govern condensate diffusion during hyperosmotic stress.
    Article Snippet: U2OS (HTB-96, ATCC) and U2OS-eGFP-DCP1A (UGD [21]) cell lines were cultured in McCoy's 5A medium (Thermo Fisher, #16600082) supplemented with 10% (v/v) fetal bovine serum (Fisher Scientific, #MT35016CV) and 20 U/ml Penicillin-Streptomycin (Invitrogen, #15140122) at 37°C in 5% CO2. .. The identity of the U2OS cell line was authenticated by the supplier (ATCC), and the U2OS-eGFP-DCP1A (UGD) stable line was verified by confirming the expression and localization of the fluorescently tagged protein. ..



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    ( A–B ) Measurement of total Orai1 levels in the plasma membrane of STX11-depleted cells by flow-cytometry. <t>U2OS</t> cells stably expressing Orai1-BBS-YFP were transduced with Scr (black) or STX11 (red) shRNA, stimulated with 1 µM TG (broken lines) or control (solid lines) and incubated with alpha-bungarotoxin alexa fluor 647 (BTX-AF647). Total Orai1-YFP levels ( A ) and BTX-AF647 binding to surface Orai1 ( B ) were measured where binding to wildtype U2OS cells was used as the background fluorescence. N=3 ( C ) Representative quantitative PCR to assess the efficiency of knockdown in shRNA-treated Orai1-BBS-YFP expressing U2OS cells from ( A–B ). Total RNA extracted from cells treated with STX11 shRNA was subjected to qPCR analysis using Taqman probes for STX11. Data were normalized to RPL30 housekeeping gene. ( D–E ) Measurement of thapsigargin (TG) induced SOCE in U2OS cells treated with STX11 RNAi in ( A–B ). ( D ) Representative averaged traces of Fura-2 calcium imaging assay performed on cells in panels ( A–B ). ( E ) Bar plot showing relative mean % SOCE ± SE from three independent experiments in ( D ). Figure 2—figure supplement 2—source data 1. Folder containing the original flow cytometry files and gating strategy for . Figure 2—figure supplement 2—source data 2. Numerical data underlying the quantitative analyses represented in .
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    Image Search Results


    ( A–B ) Measurement of total Orai1 levels in the plasma membrane of STX11-depleted cells by flow-cytometry. U2OS cells stably expressing Orai1-BBS-YFP were transduced with Scr (black) or STX11 (red) shRNA, stimulated with 1 µM TG (broken lines) or control (solid lines) and incubated with alpha-bungarotoxin alexa fluor 647 (BTX-AF647). Total Orai1-YFP levels ( A ) and BTX-AF647 binding to surface Orai1 ( B ) were measured where binding to wildtype U2OS cells was used as the background fluorescence. N=3 ( C ) Representative quantitative PCR to assess the efficiency of knockdown in shRNA-treated Orai1-BBS-YFP expressing U2OS cells from ( A–B ). Total RNA extracted from cells treated with STX11 shRNA was subjected to qPCR analysis using Taqman probes for STX11. Data were normalized to RPL30 housekeeping gene. ( D–E ) Measurement of thapsigargin (TG) induced SOCE in U2OS cells treated with STX11 RNAi in ( A–B ). ( D ) Representative averaged traces of Fura-2 calcium imaging assay performed on cells in panels ( A–B ). ( E ) Bar plot showing relative mean % SOCE ± SE from three independent experiments in ( D ). Figure 2—figure supplement 2—source data 1. Folder containing the original flow cytometry files and gating strategy for . Figure 2—figure supplement 2—source data 2. Numerical data underlying the quantitative analyses represented in .

    Journal: eLife

    Article Title: Syntaxin11 deficiency inhibits CRAC channel priming to suppress cytotoxicity and gene expression in T lymphocytes independent of membrane trafficking

    doi: 10.7554/eLife.111335

    Figure Lengend Snippet: ( A–B ) Measurement of total Orai1 levels in the plasma membrane of STX11-depleted cells by flow-cytometry. U2OS cells stably expressing Orai1-BBS-YFP were transduced with Scr (black) or STX11 (red) shRNA, stimulated with 1 µM TG (broken lines) or control (solid lines) and incubated with alpha-bungarotoxin alexa fluor 647 (BTX-AF647). Total Orai1-YFP levels ( A ) and BTX-AF647 binding to surface Orai1 ( B ) were measured where binding to wildtype U2OS cells was used as the background fluorescence. N=3 ( C ) Representative quantitative PCR to assess the efficiency of knockdown in shRNA-treated Orai1-BBS-YFP expressing U2OS cells from ( A–B ). Total RNA extracted from cells treated with STX11 shRNA was subjected to qPCR analysis using Taqman probes for STX11. Data were normalized to RPL30 housekeeping gene. ( D–E ) Measurement of thapsigargin (TG) induced SOCE in U2OS cells treated with STX11 RNAi in ( A–B ). ( D ) Representative averaged traces of Fura-2 calcium imaging assay performed on cells in panels ( A–B ). ( E ) Bar plot showing relative mean % SOCE ± SE from three independent experiments in ( D ). Figure 2—figure supplement 2—source data 1. Folder containing the original flow cytometry files and gating strategy for . Figure 2—figure supplement 2—source data 2. Numerical data underlying the quantitative analyses represented in .

    Article Snippet: Cell line ( Homo sapiens ) , U2OS cell line , ATCC , HTB-96 (RRID: CVCL_0042 ) , .

    Techniques: Clinical Proteomics, Membrane, Flow Cytometry, Stable Transfection, Expressing, Transduction, shRNA, Control, Incubation, Binding Assay, Fluorescence, Real-time Polymerase Chain Reaction, Knockdown, Analysis, Imaging

    Journal: eLife

    Article Title: Syntaxin11 deficiency inhibits CRAC channel priming to suppress cytotoxicity and gene expression in T lymphocytes independent of membrane trafficking

    doi: 10.7554/eLife.111335

    Figure Lengend Snippet:

    Article Snippet: Cell line ( Homo sapiens ) , U2OS cell line , ATCC , HTB-96 (RRID: CVCL_0042 ) , .

    Techniques:

    Integrated single‐cell landscape of human osteosarcoma. (A) Harmony‐integrated UMAP of 39,063 quality‐controlled cells colored by global cluster. (B) The same embedding colored by patient, showing cross‐patient mixing after integration. (C) Reviewed major‐cell‐type annotations displayed on the integrated UMAP; labels mark lineage centroids. (D) Relative abundance of major cell types in each patient. Colors are consistent between panels C and D.

    Journal: International Journal of Genomics

    Article Title: Integrative Single‐Cell and Bulk Transcriptomics Identifies a Proliferative Malignant State and Supports a Functional Contribution of TROAP in Osteosarcoma

    doi: 10.1155/ijog/1407947

    Figure Lengend Snippet: Integrated single‐cell landscape of human osteosarcoma. (A) Harmony‐integrated UMAP of 39,063 quality‐controlled cells colored by global cluster. (B) The same embedding colored by patient, showing cross‐patient mixing after integration. (C) Reviewed major‐cell‐type annotations displayed on the integrated UMAP; labels mark lineage centroids. (D) Relative abundance of major cell types in each patient. Colors are consistent between panels C and D.

    Article Snippet: HOS and U2OS human osteosarcoma cell lines were obtained from the American Type Culture Collection (ATCC; Manassas, Virginia, United States; HOS, CRL‐1543; U2OS, HTB‐96).

    Techniques: Single Cell

    TROAP depletion reduces clonogenic growth, overall wound closure, migration, and invasion in osteosarcoma cells. (A) Immunoblot analysis of TROAP in HOS and U2OS cells transfected with siNC, siTROAP‐1, or siTROAP‐2; beta‐actin is the loading control. (B) Colony‐formation images and colony counts. (C) Scratch‐wound images at 0 and 24 h and overall wound closure. Because the assay was not proliferation‐matched, it is not interpreted as direct migration evidence. (D) Transwell migration and invasion images and cell counts. Data are mean + /−standard deviation from three independent biological experiments. One‐way ANOVA with Dunnett′s test compared each siTROAP group with siNC; ∗∗∗ p < 0.001.

    Journal: International Journal of Genomics

    Article Title: Integrative Single‐Cell and Bulk Transcriptomics Identifies a Proliferative Malignant State and Supports a Functional Contribution of TROAP in Osteosarcoma

    doi: 10.1155/ijog/1407947

    Figure Lengend Snippet: TROAP depletion reduces clonogenic growth, overall wound closure, migration, and invasion in osteosarcoma cells. (A) Immunoblot analysis of TROAP in HOS and U2OS cells transfected with siNC, siTROAP‐1, or siTROAP‐2; beta‐actin is the loading control. (B) Colony‐formation images and colony counts. (C) Scratch‐wound images at 0 and 24 h and overall wound closure. Because the assay was not proliferation‐matched, it is not interpreted as direct migration evidence. (D) Transwell migration and invasion images and cell counts. Data are mean + /−standard deviation from three independent biological experiments. One‐way ANOVA with Dunnett′s test compared each siTROAP group with siNC; ∗∗∗ p < 0.001.

    Article Snippet: HOS and U2OS human osteosarcoma cell lines were obtained from the American Type Culture Collection (ATCC; Manassas, Virginia, United States; HOS, CRL‐1543; U2OS, HTB‐96).

    Techniques: Migration, Western Blot, Analysis, Transfection, Control, Colony Assay, Standard Deviation